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Max-Planck-Insti­tut für Infek­ti­ons­bio­lo­gie

We, the Taylor lab at the Max Planck Insti­tute for Infec­tion Bio­logy, seek to under­stand the molecu­lar mech­an­isms of how immune cells sense, trans­mit and pro­cess chem­ical inform­a­tion to make decisions. Com­bin­ing a vari­ety of syn­thetic, bio­chem­ical, molecu­lar and cell bio­lo­gical approaches allows us to invest­ig­ate these molecu­lar mech­an­isms. In the long-term, we aim to re-engin­eer cel­lu­lar sig­nal­ing sys­tems to con­trol cell beha­vior and func­tion.

Mas­ter thesis or intern­ship – resolv­ing innate immune sig­nal­ing

We are look­ing for a mas­ter stu­dent to work on the research pro­ject

„Prox­im­ity Pro­teo­m­ics to resolve Toll-Like Receptor sig­nal­ing in space and time”

The pro­ject
Toll-like Recept­ors (TLR) respond to the molecu­lar sig­na­tures of microbes and are crit­ical to launch­ing an innate immune response. Clas­sical bio­chem­istry and genet­ics have revealed the pro­tein net­work required for sig­nal propaga­tion; however, these tech­niques gen­er­ally focused on sig­nal­ing out­puts and ana­lysis of pro­tein com­plexes isol­ated by immun­o­precip­it­a­tion. Con­sequently, there is no spa­ti­otem­poral data on how the TLR sig­nal­ing net­works remodel in time and space within the cel­lu­lar cyto­plasm. This pro­ject seeks to address this lack of know­ledge using BioID pro­teo­m­ics to char­ac­ter­ize the TLR sig­nal­ing pro­tein net­work, its sub­cel­lu­lar loc­al­iz­a­tion, and its evol­u­tion over time.

Work­ing field:

Your tasks
  • Cell cul­ture main­ten­ance of dif­fer­ent cell types
  • Dif­fer­en­ti­ation exper­i­ments of semi-immor­tal­ized bone mar­row cells (hoxB8)
  • Recon­sti­t­u­tion of TLR4 sig­nal­ing in HEK cells
  • Prox­im­ity labeling exper­i­ments upon stim­u­la­tion
  • Pro­tein bio­chem­istry e.g. pro­tein puri­fic­a­tion and west­ern blots

Require­ments:

Qual­i­fic­a­tion pro­file
  • Mas­ter stu­dent in Bio­chem­istry, Bio­logy, Bio­tech­no­logy or related
  • Min­imum of 6 months (start 1st Decem­ber 2020)
  • Strong the­or­et­ical know­ledge in bio­chem­istry, cell bio­logy, molecu­lar bio­logy and immun­o­logy
  • Prac­tical exper­i­ence in cell cul­ture and basic bio­chem­istry meth­ods
  • Work­ing in an inde­pend­ent mat­ter, motiv­ated and resi­li­ent
  • Good team spirit and solid com­mu­nic­a­tion skills in Eng­lish

What we of­fer:

  • Inter­na­tional, inter­dis­cip­lin­ary and motiv­ated team
  • Pos­sible par­ti­cip­a­tion at a vari­ety of sem­inars
  • Dili­gent incor­por­a­tion and super­vi­sion by a dir­ect con­tact per­son (PhD stu­dent)

How to ap­ply:

Please send your applic­a­tion (includ­ing a CV and cer­ti­fic­ates) in one single doc­u­ment to
Mar­cus Taylor taylor@mpiib-berlin.mpg.de and
Fenja Ger­pott gerpott@mpiib-berlin.mpg.de
until 30.10.2020.