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Freie Uni­ver­si­tät Ber­lin - Fach­be­reich Bio­lo­gie, Che­mie, Phar­ma­zie - Insti­tut für Che­mie und Bio­che­mie Mus­cu­los­keletal Deve­lop­ment and Rege­ne­ra­tion Group

PhD stu­dent (m/f/d)

65%-part-time job | Limi­ted to 3 years | Ent­gelt­gruppe 13 TV-L FU | Refence code: Adipo_2020

Work­ing field:

A PhD stu­dent posi­tion is avail­able in the Mus­cu­los­keletal Deve­lop­ment and Rege­ne­ra­tion Lab (https://www.bcp.fu-berlin.de/en/chemie/biochemie/research-groups/stricker-group/index.html) at the Insti­tute of Che­mi­stry and Bio­che­mi­stry of the Freie Uni­ver­si­tät Ber­lin. We are a dyna­mic young group inte­rested in tran­scrip­tio­nal mecha­nisms con­trol­ling embryo­nic mesen­chy­mal pro­ge­ni­tor- and adult mesen­chy­mal stem cell dif­fe­ren­tia­tion. The posi­tion will be part of a DFG-fun­ded pro­ject addres­sing tran­scrip­tio­nal and epi­ge­ne­tic mecha­nisms of adi­po­ge­nic dif­fe­ren­tia­tion in deve­lop­ment and the adult. We use cell models in com­bi­na­tion with, where appro­priate, mouse models. 
Our lab is part of the vibrant inter­di­sci­pli­nary sci­en­ti­fic land­s­cape of Ber­lin with its uni­que col­la­bo­ra­tive envi­ron­ment. We are part of the ECRT (Ein­stein Cen­ter for Rege­ne­ra­tive The­ra­pies), BSRT (Ber­lin-Bran­den­burg School for Rege­ne­ra­tive The­ra­pies) and Myo­Grad (Inter­na­tio­nal Rese­arch Trai­ning Group for Myo­logy). The PhD pro­ject can be inte­gra­ted into one of several Ber­lin-based gra­duate trai­ning schools that offer widespread edu­ca­tion and sup­port.

Job descrip­tion:
Your task will be to con­duct and deve­lop a rese­arch pro­ject in close feed­back with the lab’s PI. Methods that will be used include: FACS-Extrac­tion of pri­mary cells from mice for ana­ly­sis and cul­ti­va­tion; Estab­lish­ment and main­ten­ance of pri­mary cells and cell lines; Estab­lish­ment of explant cul­tures from mouse embryos; trans­fec­tion and siRNA media­ted knock­down in cell lines; Ana­ly­sis of cell lines and mouse tis­sues by RNA-Seq, ChIP-Seq and ATAC-Seq; Bio­in­for­ma­tic eva­lua­tion of sequen­cing data; Ana­ly­sis of gene­ti­cally modi­fied mouse models (his­to­logy, RNA-in-situ hybri­di­sa­tion; immu­no­la­be­ling); FACS ana­ly­sis of adult stromal cells; Plan­ning, app­li­ca­tion for, and imple­men­ta­tion of work on trans­ge­nic mouse models. You will be able to pre­sent your work in natio­nal and inter­na­tio­nal con­fe­ren­ces, and are expec­ted to con­tri­bute to manu­script and grant app­li­ca­tion deve­lop­ment.

Require­ments:

Requi­re­ments:
Mas­ter’s degree in Bio­che­mi­stry, Bio­logy or rela­ted disci­pli­nes.

Desi­ra­ble:
Desi­red skills:
Good com­mu­ni­ca­tion skills are pre­re­qui­site to work in our inter­na­tio­nal team.
Expe­ri­ence in mole­cu­lar bio­logy, cell bio­logy, and in tran­scrip­tio­nal and epi­ge­ne­tic regu­la­tion - both prac­ti­cal and theo­re­ti­cal.
Basic know­ledge in the area of mesen­chy­mal cell dif­fe­ren­tia­tion in the embryo, espe­ci­ally myo­ge­ne­sis and adi­po­ge­ne­sis, is an advan­tage; also basic know­ledge of the ana­tomy and his­to­logy of the mouse embryo.
Expe­ri­ence in next-genera­tion sequen­cing-based methods (RNA-Seq, ChIP-Seq, ATAC-Seq) as well as bio­in­for­ma­tic eva­lua­tion and inter­pre­ta­tion of the data, also with the help of online data­ba­ses.
Expe­ri­ence in the app­li­ca­tion of flow cyto­me­try and fluo­re­scence micro­scopy inclu­ding con­fo­cal micro­scopy, as well as in pro­grams for the eva­lua­tion of image data.
Expe­ri­ence in cell cul­ture, espe­ci­ally with pri­mary cells and tis­sue explants; app­li­ca­tion of cell dif­fe­ren­tia­tion assays; iso­la­tion of stroma cells from adi­pose tis­sue; know­ledge of orga­noid models would be an advan­tage.
Expe­ri­ence and know­ledge - both prac­ti­cal and theo­re­ti­cal - in dealing with trans­ge­nic mouse models, inclu­ding Cre/loxP-based lineage tra­cing models, and the wil­ling­ness to work with them, is par­ti­cu­larly desi­ra­ble. Prac­ti­cal expe­ri­ence in the ana­ly­sis of mouse models using his­to­lo­gi­cal methods (fro­zen sec­tions, his­to­lo­gi­cal stai­ning), as well as using RNA-in-situ hybri­di­za­tion and immu­nostai­ning on tis­sue sec­tions are advan­ta­ge­ous. Know­ledge of plan­ning and app­ly­ing for ani­mal expe­ri­ments as well as being in pos­ses­sion of a FEL­ASA-B cer­ti­fi­cate are an advan­tage.
Addi­tio­nal qua­li­fi­ca­ti­ons: suc­cess­ful acqui­si­tion of third-party fun­ding; proof of sci­en­ti­fic manu­scripts; capa­city for team­work and coope­ra­tion; good pre­sen­ta­tion and wri­ting skills; expe­ri­ence in the super­vi­sion of bache­lor or mas­ter stu­dents.

For fur­ther infor­ma­tion, please con­tact Prof. Dr. Sig­mar Stri­cker (sigmar.stricker@fu-berlin.de / +49 30 838 75799).

How to ap­ply:

All app­li­ca­tion quo­ting the refe­rence code should be addres­sed as an e-mail no later than Octo­ber 26th, 2020 to Prof. Dr. Sig­mar Stri­cker: sigmar.stricker@fu-berlin.de or pos­tal to

Freie Uni­ver­si­tät Ber­lin
Fach­be­reich Bio­lo­gie, Che­mie, Phar­ma­zie
Insti­tut für Che­mie und Bio­che­mie
Mus­cu­los­keletal Deve­lop­ment and Rege­ne­ra­tion Group
Mr. Prof. Dr. Sig­mar Stri­cker
Thiel­al­lee 63
14195 Ber­lin (Dah­lem)

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