Charité-Universitätsmedizin Berlin - Berlin-Brandenburg Center for Regenerative Therapies
Das BCRT wurde 2006 als Allianz der Charité-Universitätsmedizin Berlin und der Helmholtz-Gemeinschaft gegründet. Mehr als 250 Mitarbeiter – Ärzte, Naturwissenschaftler, Translationsexperten, Ingenieure, Doktoranden und technische Mitarbeiter – forschen und arbeiten an den zwei Standorten des BCRT: am Institutsgebäude Süd des Campus Virchow-Klinikum der Charité und am Campus Teltow des Instituts für Biomaterialforschung der Helmholtz-Zentrums Geesthacht GmbH.
Looking for Bioengineering/Physics Student to Perform Master Thesis (Abschlussarbeit/Pflichtpraktikum)
for a project “Ultrasound-Enforced Engineering of 3D Tissue Scaffolds for Regeneration of Musculoskeletal System.”
The uniform seeding of the cells in a scaffold does not necessarily reflect native tissue conditions. In order to provide proper functioning of the engineered tissue, spatially cell/ECM patterned constructs are often required. In the offered here study we are aiming to develop a bio-acoustic levitational assembly (BAL) technology for a patterned cell-seeding in 3D scaffolds, employing ultrasound standing waves, which has been previously described by our collaborators from Lyon. Additionally, the BAL technique will be combined with our Focused Low-Intensity Pulsed Ultrasound (FLIPUS) in-vitro set-up for spatial- and time-controlled release of growth factors enhancing tissue regeneration process. The successful patterning of the 3D scaffold will be further visualized by high-frequency ultrasound and multiphoton confocal microscopy. Biochemical assays are to be performed to evaluate cellular activities and efficacy of the released growth factor.
The project’s agenda will include:
Building a BAL set-up
Optimization of the assembly acoustic parameters
Optimization of FLIPUS acoustic parameters
Some knowledge in biochemistry and tissue cell culture is favorable, but not required.
What we offer:
We are a multinational team of interdisciplinary scientists, working on novel musculoskeletal tissue imaging and regeneration approaches. For this challenging study, we would like to have a Master Student who has strong engineering and/or physics background and is excited to work on clinically significant projects.
How to apply:
Please send your CV to email@example.com if you are interested in this project and do not hesitate to ask if you have any questions!