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Cha­rité-Uni­ver­si­täts­me­di­zin Ber­lin - Ber­lin-Branden­burg Cen­ter for Regen­er­at­ive Ther­apies

Das BCRT wurde 2006 als Alli­anz der Cha­rité-Uni­ver­si­täts­me­di­zin Ber­lin und der Helm­holtz-Gemein­schaft gegrün­det. Mehr als 250 Mit­ar­bei­ter – Ärzte, Natur­wis­sen­schaft­ler, Trans­la­ti­ons­ex­per­ten, Inge­nieure, Dok­to­ran­den und tech­ni­sche Mit­ar­bei­ter – for­schen und arbei­ten an den zwei Stand­or­ten des BCRT: am Insti­tuts­ge­bäude Süd des Cam­pus Vir­chow-Kli­ni­kum der Cha­rité und am Cam­pus Tel­tow des Insti­tuts für Bio­ma­te­ri­al­for­schung der Helm­holtz-Zen­trums Geest­hacht GmbH.

Look­ing for Bioen­gin­eer­ing/Phys­ics Stu­dent to Per­form Mas­ter Thesis (Abschlus­sarbeit/Pflicht­prak­tikum)

for a pro­ject “Ultra­sound-Enforced Engin­eer­ing of 3D Tis­sue Scaf­folds for Regen­er­a­tion of Mus­cu­lo­skeletal Sys­tem.”

Work­ing field:

The uni­form seed­ing of the cells in a scaf­fold does not neces­sar­ily reflect nat­ive tis­sue con­di­tions. In order to provide proper func­tion­ing of the engin­eered tis­sue, spa­tially cell/ECM pat­terned con­structs are often required. In the offered here study we are aim­ing to develop a bio-acous­tic lev­it­a­tional assembly (BAL) tech­no­logy for a pat­terned cell-seed­ing in 3D scaf­folds, employ­ing ultra­sound stand­ing waves, which has been pre­vi­ously described by our col­lab­or­at­ors from Lyon. Addi­tion­ally, the BAL tech­nique will be com­bined with our Focused Low-Intens­ity Pulsed Ultra­sound (FLIPUS) in-vitro set-up for spa­tial- and time-con­trolled release of growth factors enhan­cing tis­sue regen­er­a­tion pro­cess. The suc­cess­ful pat­tern­ing of the 3D scaf­fold will be fur­ther visu­al­ized by high-fre­quency ultra­sound and mul­ti­photon con­focal micro­scopy. Bio­chem­ical assays are to be per­formed to eval­u­ate cel­lu­lar activ­it­ies and effic­acy of the released growth factor.

Require­ments:

The pro­ject’s agenda will include:
  • Build­ing a BAL set-up
  • Optim­iz­a­tion of the assembly acous­tic para­met­ers
  • Optim­iz­a­tion of FLIPUS acous­tic para­met­ers
  • Mat­lab pro­gram­ming
Some know­ledge in bio­chem­istry and tis­sue cell cul­ture is favor­able, but not required.

What we of­fer:

We are a mul­tina­tional team of inter­dis­cip­lin­ary sci­ent­ists, work­ing on novel mus­cu­lo­skeletal tis­sue ima­ging and regen­er­a­tion approaches. For this chal­len­ging study, we would like to have a Mas­ter Stu­dent who has strong engin­eer­ing and/or phys­ics back­ground and is excited to work on clin­ic­ally sig­ni­fic­ant pro­jects.

How to ap­ply:

Please send your CV to regina.puts@charite.de if you are inter­ested in this pro­ject and do not hes­it­ate to ask if you have any ques­tions!