Charité - Universitätsmedizin Berlin - Institute for Experimental Pediatric Endocrinology/ CC17
The Charité - Universitätsmedizin Berlin is a joint medical faculty, which serves both Freie Universität Berlin and Humboldt Universität zu Berlin. As one of the largest university hospitals in Europe with an important history, it plays a leading role in research, teaching and clinical care. The Charité university hospital has also made a name for itself as a modern business with certifications in the medical, clinical and management fields.
Post doctoral scientist
A postdoctoral position is available starting 01.09.2021 at the Institute for Experimental Pediatric Endocrinology at the Charité Universitätsmedizin Berlin (AG Kühnen). This position is associated to a DFG grant KU 2673/6-1.
The primary focus of our group’s research is on rare endocrine diseases. Special topics include genetic and epigenetic mechanisms of central regulation of body weight and thyroid hormone action on brain development. To analyze the impact of genetic and epigenetic variants on hormonally-regulated molecular pathways, our group’s translational research prioritizes human experimental models based on human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC).
Your area of responsibility
The candidate will work in a DFG funded project. This project specifically aims at characterizing central signaling cascades, which are playing a pivotal role for body weight regulation. Thereby, new therapeutic strategies should be elucidated. This aim will be addressed by analyzing differentiated hiPSC lines (healthy, patient-derived and gene-edited hiPSC) and hESC based 2-dimensional models. The candidate will be expected to investigate the development of critical methylome signatures and to functional characterize critical central pathways.
Core techniques relevant to the project include:
2D hESC neuronal cultures and hiPSC-derived brain organoids
CRISPR/Cas9 gene editing
Immunofluorescence staining and single molecule fluorescent in situ hybridization (RNAscope)
Single cell RNA sequencing (scRNA-seq)
methylome analyses by pyrosequencing and post-bisulfite adapter tagging (PBAT), TWGBS
Additional epigenomic techniques relevant for this project will include Chromatin Immunoprecipitation Sequencing (ChIP-seq), Assays for Transposase-Accessible Chromatin (ATAC-seq)
Scientific staff is given sufficient time to carry out their own scientific work in accordance with their employment relationship.
We are looking for an enthusiastic, talented and motivated post-doctoral scientist with a positive mindset.
Candidates with experimental experience in developmental research and a strong interest in working with stem cell-based models are encouraged to apply.
Candidates should hold a PhD degree in cell/molecular/developmental/stem cell biology or similar, with a track-record of successful scientific work.
Previous experience in analyzing neuronal tissue by means of multidimensional microscopy, advanced image analysis and single cell RNA sequencing are considered an advantage.
Enhanced skills in bioinformatics (single cell data analyses) are highly appreciated.
The ability to work in a highly collaborative environment both independently and as part of a team is essential.
The candidate should also have the motivation to participate in the training and supervision of young scientists (e.g. doctoral students) in addition to the scientific work.
Excellent English communication skills, both oral and written, are required for a successful candidate.