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Max Planck Insti­tute of Col­loids and Inter­faces - Depart­ment of Bio­ma­ter­i­als

The Depart­ment of Bio­ma­ter­i­als focuses on inter­dis­cip­lin­ary research in the field of bio­lo­gical and bio­mi­metic mater­i­als. The emphasis is on under­stand­ing how the mech­an­ical or other phys­ical prop­er­ties are gov­erned by struc­ture and com­pos­i­tion and how they adopt to envir­on­men­tal con­di­tions.

MSc thesis: Chori­oal­lan­toic mem­brane (CAM) assay for the study of mul­tiple myel­oma

Devel­op­ment of an altern­at­ive, less sen­tient in vivo model (CAM assay) to study the effect of mul­tiple myel­oma can­cer cells in the bone mat­rix microen­vir­on­ment.

Work­ing field:

Back­ground: Bio­logy, Bio­medi­cine, Bio­tech­no­logy or Bio­med­ical Engin­eer­ing

Require­ments:

You should have a back­ground in bio­logy, bio­medi­cine, bio­med­ical engin­eer­ing, bio­tech­no­logy or sim­ilar, and strong interest in can­cer bio­logy. Pre­vi­ous exper­i­ence with cell cul­ture will be highly valu­able, but not essen­tial. The pro­ject will be car­ried out at the Max Planck Insti­tute of Col­loids and Inter­faces in the group of Dr Amaia Cipitria together with Dr Inés Moreno.

What we of­fer:

Mul­tiple myel­oma (MM) is a type of blood can­cer in which plasma cells grow aber­rantly and pro­duce abnor­mal anti­bod­ies. In the clinic, MM patients show lesions in the skel­eton which do not heal, even when the patient is in com­plete remis­sion. MM cells mainly reside in the bone mar­row and alter bone homeo­stasis by activ­at­ing bone resorp­tion and inhib­it­ing bone form­a­tion. We are inter­ested in under­stand­ing how these can­cer cells alter the bio­phys­ical prop­er­ties of the bone extra­cel­lu­lar mat­rix. The pro­ject will involve cell cul­ture of human MM cell lines to study their inter­ac­tion and effects with the extra­cel­lu­lar mat­rix of embryonic chick femurs. In the first part of the pro­ject you will cul­ture femurs from chick embryos in vitro (organ­o­typ­ic­ally) together with MM cells. In a second part of the pro­ject you will use the chori­oal­lan­toic mem­brane (CAM) assay as a less sen­tient, refine­ment model (3Rs) to exam­ine the inter­ac­tion of can­cer cells with the bone tis­sue in vivo. Explor­at­ory ana­lysis will involve his­to­logy to loc­al­ize can­cer cells in the tis­sue and micro com­puted tomo­graphy to eval­u­ate 3D changes in the bone struc­ture.

How to ap­ply:

Please send your applic­a­tion includ­ing a motiv­a­tion let­ter, your CV and a tran­script of your uni­versity record to these two email addresses: ines.moreno@mpikg.mpg.de and amaia.cipitria@mpikg.mpg.de Please indic­ate “Mas­ter thesis –CAM assay and mul­tiple myel­oma” in the sub­ject line. The pro­ject can start at the begin­ning of March 2020. The work­ing lan­guage is Eng­lish.
Dr Amaia Cipitria (Emmy Noether Group Leader) and Dr Inés Moreno (Hum­boldt Postdoc­toral researcher), Dept. of Bio­ma­ter­i­als, Max Planck Insti­tute of Col­loids and Inter­faces, Golm, Pots­dam.