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Offer 274 out of 591 from 13/05/22, 13:48

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Cha­rité - Uni­ver­si­täts­me­di­zin Ber­lin - CC14 / Dept. of Radiooncology and Radiotherapy / Translational Radiooncology Laboratory

In this DFG -funded research project “Assessing clonal evolution in head and neck squamous cell carcinoma and its contribution to tumor progression and treatment” resistance we seek to decipher biological processes promoting radioresistance using clonal barcoding techniques for ultra-deep resolution of clonal dynamics ex vivo and in vivo combined with state-of-the-art genomic and transcriptomics profiling approaches.
We expect that a precise understanding of the biology of therapy-resistant tumor cell clones and their molecular characteristics will enable us to develop new treatment modalities for therapy-resistant tumors.

Scientific Researcher

Charité Campus Mitte

Working field:

We are seeking a highly motivated doctoral student who will apply cutting-edge techniques for cellular barcoding and molecular profiling to understand the determinants of cancer therapy resistance.
In the project, we will use (i) extensively characterized, patient-derived HNSCC in-vivo models and three-dimensional (3D) cultures; (ii) advanced lentiviral vectors for cellular barcoding using novel barcode libraries compatible with single-cell RNA sequencing and designed for high-precision clonal tracking; (iii) many years of experience with the CRISPR-Cas9 gene scissors to elucidate the causal relationships between genetics and biology of tumor cells.

  • The successful applicant will generate stable replica cultures of barcoded organoid models from HNSCC patients.
  • He/She will analyse treatment-induced changes in the clonal spectrum together with detailed characterisation of resistant subclones using bulk and single cell DNA/RNA sequencing.
  • The student will identify drivers of therapy resistance ex vivo and in vivo, using suitable functional analyses to validate therapeutic candidates.

Training will be offered in organoid culture techniques, lentiviral transduction, barcode sequencing and bioinformatic analysis.

Requirements:

  • Applicants must hold a master's degree in biology, biochemistry, biotechnology, or a related field;
  • The successful candidates are highly motivated, ambitious, self-driven and hard-working individuals with excellent grades;
  • Candidates should enjoy working in an international team;
  • An excellent written and oral command of English is essential;
  • Candidates are expected to have excellent presentation and writing skills;
  • The ideal candidate should have strong interest in cell/tumor biology and state of the art functional genomic approaches;
  • Experience in basic molecular biology techniques (DNA, RNA, Protein) and next generation sequencing is beneficial;
  • Computational knowledge to handle and analyse NGS data (R, Bioconductor) is an advantage
  • Applications should include a motivation letter, CV, copy of certificates, expected availability date, a complete list of publications, and 2 references

Scientific staff is given sufficient time to carry out their own scientific work in accordance with their employment relationship.

How to apply:

Bitte sen­den Sie sämt­li­che Bewer­bungs­un­ter­la­gen, wie z.B. Anschrei­ben, Lebens­lauf, Zeug­nisse, Urkun­den usw. als PDF oder Bild­da­tei unter Angabe der Kenn­zif­fer an fol­gende Adresse:

Cha­rité - Uni­ver­si­täts­me­di­zin Ber­lin
Kli­nik für Radioon­ko­lo­gie und Strah­len­the­ra­pie
Prof. Dr. Inge­borg Tin­ho­fer-Keil­holz
Cha­rité­platz 1, 10117 Ber­lin

E-Mail­adresse zum Ein­sen­den der Bewer­bungs­un­ter­la­gen:
vanessa-deborah.sachse@charite.de