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Tech­ni­sche Uni­ver­sität Ber­lin - Faculty II - Institute of Chemistry / Bioenergetics

Research Assistant - 0.5 working time - salary grade E13 TV-L Berliner Hochschulen - 1st
qualification period (PhD candidate)

Work­ing field:

Research on structure-function relationships of carotenoid-binding proteins, teaching obligations (2 SWS, hours per semester week at 0.5 part-time) in laboratory practical couses and tutorials in Physical Chemistry
  • Generation of cDNA constructs for protein and carotenoid production in bacteria by molecular-biological methods, site-directed mutagenesis, recombinant PCR
  • Carotenoid and protein production in bacteria, chromatographic purification and analytics
  • Mass spectrometry of carotenoids and carotenoid-binding proteins
  • Protein expression with template-directed incorporation of non-natural amino acids
  • Spectroscopic studies of carotenoid-binding proteins
  • Probe preparation and analysis with NMR (structure determination)
  • Teaching obligations in laboratory practical courses and tutorials (2 SWS, hours per semester week at 0.5 part-time) in Physical Chemistry
  • Writing/editing of scientific manuscripts for publication, conference and project reports

Require­ments:

  • Successfully completed university degree (Master, Diplom or equivalent) in chemistry with major subjects (bio)-physical chemistry and biological chemistry
  • Extensive experience in molecular biology, protein biochemistry and bioanalytical techniques (FPLC, HPLC, SEC, HIC, ESI-MS, LC-MS)
  • Expertise in spectroscopic techniques (UV-Vis absorption spectroscopy, fluorescence spectroscopy, fluorescence lifetime spectroscopy, CD spectroscopy)
  • Firm expertise in handling complex computational data analysis tools for evaluating spectroscopic data
  • Practical experience with Synthetic Biology methods (template-directed incorporation of non-canonical amino acids into proteins)
Optional:
Experience with computational modeling of complex reaction kinetics with rate equation models, fluent German and English (oral and written), expertise in university teaching and education, especially teaching formats of research-based learning.

How to ap­ply:

Please send your application with the reference number and the usual documents (letter of motivation, CV, list of publications, if applicable, all combined in a single pdf file, max. 5 MB) by email to Prof. Dr. Thomas Friedrich (friedrich@chem.tu-berlin.de).

By submitting your application via email you consent to having your data electronically processed and saved. Please note that we do not provide a guaranty for the protection of your personal data when submitted as unprotected file. Please find our data protection notice acc. DSGVO (General Data Protection Regulation) at the TU staff department homepage: https://www.abt2-t.tu-berlin.de/menue/themen_a_z/datenschutzerklaerung/ or quick access 214041.

To ensure equal opportunities between women and men, applications by women with the required qualifications are explicitly desired. Qualified individuals with disabilities will be favored. The TU Berlin values the diversity of its members and is committed to the goals of equal opportunities.

Technische Universität Berlin - Der Präsident - , Fakultät II, Institut für Chemie, FG Bioenergetik, Prof. Dr. Friedrich, Sekr. PC 14, Straße des 17. Juni 135, 10623 Berlin