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An­ge­bot 196 von 316 vom 07.10.2020, 00:00

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B-CRT - Tis­sue Immun­o­logy Lab

Regen­er­at­ive medi­cine – this is our pas­sion. The BCRT was estab­lished as an inter­dis­cip­lin­ary trans­la­tional cen­ter with the goal of enhan­cing endo­gen­ous regen­er­a­tion by cells, bio­ma­ter­i­als, and factors which can be used to develop and imple­ment innov­at­ive ther­apies and products. The primary focus of the BCRT is on dis­eases of the immune sys­tem, the mus­cu­lo­skeletal sys­tem, the car­di­ovas­cu­lar sys­tem and in the field of tis­sue homeo­stasis and cach­exia. At the BCRT clini­cians and research­ers are work­ing closely together on the medi­cine of the future: Tar­geted, per­son­al­ized medi­cine that depends on the early recog­ni­tion of patients' indi­vidual heal­ing poten­tial.

Mas­ter thesis: Tis­sue mod­els for study­ing human immune responses

Work­ing field:

Loc­a­tion mat­ters – this is also the case for the immune sys­tem. Many immune cells con­tinu­ously migrate and are trans­por­ted via blood and lymph to sur­veil dif­fer­ent parts of the body. However, the immune reac­tion against the patho­gen or tumor is hap­pen­ing loc­ally – in lymph nodes or the par­en­chyma of the affected tis­sue.
The goal of our lab at the BIH-Cen­ter for Regen­er­at­ive Ther­apies is to dis­cover how human immune cells migrate into tis­sues, how they are reg­u­lated loc­ally and release their effector mech­an­isms. To ana­lyze these events, we use and develop advanced in vitro mod­els that recapit­u­late the com­plex in vivo net­work of cells, sol­uble factors and extra­cel­lu­lar mat­rix.

Require­ments:

  • Suc­cess­ful bach­elor’s degree
  • enrolled in Bio­tech­no­logy, Bio­logy, Bio­chem­istry or any com­par­able sub­ject area
  • Hands on exper­i­ence with cell cul­ture would be bene­fi­cial
  • Very good writ­ten and oral Eng­lish skills

What we of­fer:

In this spe­cific pro­ject, the goal is to develop a human alve­olar model, that can be used to study immune cell migra­tion. You will determ­ine the neces­sary con­di­tions (extra­cel­lu­lar mat­rix, medium, mem­brane sup­port) to grow a novel human pneumo­cyte cell line in liquid-liquid and air-liquid cul­ture and verify the cel­lu­lar iden­tity and bar­rier form­a­tion. Sub­sequently, dif­fer­ent immune cell pop­u­la­tions can be tested on this lung epi­thelial model.

You will be trained in the fol­low­ing tech­niques:
  • cell cul­ture of pneumo­cytes in liquid-liquid and air-liquid cul­ture
  • micro­scopy
  • isol­a­tion of primary immune cells
  • multi-color flow cyto­metry

How to ap­ply:

For more inform­a­tion please con­tact Dr. Regina Stark (regina.stark@charite.de)

tis­sue immun­o­logy lab
BCRT/ Char­ité
Augusten­bur­ger Platz 1
13353 Ber­lin