Regenerative medicine – this is our passion. The BCRT was established as an interdisciplinary translational center with the goal of enhancing endogenous regeneration by cells, biomaterials, and factors which can be used to develop and implement innovative therapies and products. The primary focus of the BCRT is on diseases of the immune system, the musculoskeletal system, the cardiovascular system and in the field of tissue homeostasis and cachexia. At the BCRT clinicians and researchers are working closely together on the medicine of the future: Targeted, personalized medicine that depends on the early recognition of patients' individual healing potential.
Master thesis: Tissue models for studying human immune responses
Location matters – this is also the case for the immune system. Many immune cells continuously migrate and are transported via blood and lymph to surveil different parts of the body. However, the immune reaction against the pathogen or tumor is happening locally – in lymph nodes or the parenchyma of the affected tissue.
The goal of our lab at the BIH-Center for Regenerative Therapies is to discover how human immune cells migrate into tissues, how they are regulated locally and release their effector mechanisms. To analyze these events, we use and develop advanced in vitro models that recapitulate the complex in vivo network of cells, soluble factors and extracellular matrix.
Successful bachelor’s degree
enrolled in Biotechnology, Biology, Biochemistry or any comparable subject area
Hands on experience with cell culture would be beneficial
Very good written and oral English skills
What we offer:
In this specific project, the goal is to develop a human alveolar model, that can be used to study immune cell migration. You will determine the necessary conditions (extracellular matrix, medium, membrane support) to grow a novel human pneumocyte cell line in liquid-liquid and air-liquid culture and verify the cellular identity and barrier formation. Subsequently, different immune cell populations can be tested on this lung epithelial model.
You will be trained in the following techniques:
cell culture of pneumocytes in liquid-liquid and air-liquid culture