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Angebot 152 von 507 vom 24.11.2023, 17:23

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Max Planck Insti­tute of Col­loids and Inter­faces

We aim to understand how biophysical and biochemical properties of native extracellular matrix and synthetic biomaterials guide cell response in tissue regeneration, cancer dormancy and bone metastasis

PhD position "Biocatalytic materials for in vitro protein synthesis with applications in regenerative medicine"

English

PhD position between Biogipuzkoa and CIC biomaGUNE in San Sebastián, Spain

The Cipitria Lab - Bioengineering in Regeneration and Cancer located at the Instituto de Investigación Sanitaria Biogipuzkoa, San Sebastian, Spain (https://cipitrialab.com; amaia.cipitria@biodonostia.org) is looking for a PhD student to join our interdisciplinary team. Research in our lab aims to understand how biophysical and biochemical properties of native extracellular matrix and synthetic biomaterials guide cellular response in tissue regeneration, breast cancer dormancy and bone metastasis.

This project will be carried out in collaboration with the Heterogeneous Biocatalysis Lab (https://flg802.wixsite.com/flopezgallego; flopez@cicbiomagune.es) led by Dr. Fernando López at CIC biomaGUNE. This group works on the development of enzymatic systems and cellular machineries for in vitro biotechnological applications.

Working field:

Profile:
Background in biotechnology, chemistry, biochemistry, biomedical engineering, chemical engineering or similar. Knowledge in hydrogel fabrication and cell culture will be an asset.

Research project:
The objective of the project is the development of biocatalytic materials for in vitro synthesis of proteins with applications in regenerative medicine.

First, the expression of proteins of therapeutic interest will be evaluated in vitro by trapping the transcription/translation machinery in hydrogels (Benítez-Mateos, ACS Synth Biol 2020). Cytokines such as interleukin-2 (IL-2), cell adhesion proteins such as cadherin type II and growth factors such as bone morphogenic protein 2 (BMP-2) will be investigated.

The diffusion kinetics of cytokines and growth factors in alginate-based 3D hydrogels and scaffolds will be analyzed. The physical and/or chemical crosslinking of alginates, different degree of crosslinking and degradation properties (Cipitria et al., Acta Biomater, 2017; Lueckgen et al., Biomaterials 2018; Lueckgen et al., Biomaterials 2019, Garrido et al., Biomater Adv 2023), will determine the mesh size, mechanical characteristics and diffusion of biomolecules.

Finally, the biological functionality of these cytokines and growth factors will be evaluated in cell cultures in vitro and in pre-clinical animal models by means of different molecular and cellular biology techniques (i.e. western blot, histology).

Requirements:

Master's degree (or currently completing the degree) in a relevant area of biotechnology, chemistry, biochemistry, biomedical engineering, chemical engineering or related areas is required. Experience in the field of biomaterials, hydrogels and cell culture is highly desirable.

The candidate is expected to have an interest in multidisciplinary work and motivation to learn from a diversity of scientific areas. Good interpersonal skills are required, as well as written and oral communication skills in English.

What we offer:

Duration:
We offer a PhD student contract with a duration of 18 months in Biogipuzkoa and 18 months in CIC biomaGUNE.

How to apply:

For the application, please send a single PDF file of maximum size 5 MB by e-mail to amaia.cipitria@biodonostia.org and flopez@cicbiomagune.es, including a motivation letter describing your research experience and interests, your CV, complete academic record and contact details of 2-3 references.