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An­ge­bot 298 von 619 vom 11.09.2021, 16:20

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Cha­rité - Neurowis­senschaft­liches Forschung­szen­trum

We are an inter­di­sci­pli­nary lab at the Neu­ro­sci­ence Rese­arch Cen­ter

Fish Trans­gen­esis Tech­ni­cian

Work­ing field:

Join us in estab­lish­ing Dan­ion­ella, a mini­ature singing fish, as a model organ­ism in neur­os­cience. Our lab is part of the Ein­stein Cen­ter for Neur­os­ciences and is loc­ated on the Char­ité and Hum­boldt Uni­versity cam­pus in the cen­ter of Ber­lin.

Main activ­it­ies
  • Help develop a new model organ­ism by coordin­at­ing trans­gen­esis efforts
  • Estab­lish trans­genic fish lines
  • Microin­jec­tion into single-cell stage fish embryos (Tol2 or CRISPR/Cas9)
  • Screen­ing and breed­ing of trans­genic fish
  • Main­ten­ance and char­ac­ter­iz­a­tion of fish lines
  • Basic molecu­lar bio­logy tasks (PCR, gel elec­tro­phoresis, DNA/RNA isol­a­tion)

Your pro­file
  • An under­gradu­ate degree in life sci­ences and/or train­ing as tech­ni­cian
  • Sev­eral years of prac­tical exper­i­ence with trans­gen­esis (prefer­ably in zebrafish)
  • Exper­i­ence with basic molecu­lar bio­logy tech­niques
  • Self-motiv­ated, organ­ized, effi­cient, sci­ence-driven and a team worker
  • Can­did­ates must be able to com­mu­nic­ate effect­ively in Eng­lish

Envir­on­ment & salary
  • Live in a global city that wel­comes for­eign­ers
  • Fam­ily friendly city: free day­care, kinder­garten and school
  • Salary level E9 (annual salary in the range of ~45 k€, depend­ing on level of qual­i­fic­a­tion)

Please email your applic­a­tion (cover let­ter; CV; con­tact details for ref­er­ences; cer­ti­fic­ates / tran­scripts) as one PDF to jlab@charite.de and include "[trans­gen­esis2021]" in the email sub­ject. See http://jlab.berlin for more inform­a­tion.